Our laboratory studies persister cells responsible for tolerance to antibiotics, uncultured bacteria of the environment and the microbiome, and works on drug discovery.

 

 

Kim Lewis, Ph.D.

 

 

Contact

Room 306C Mugar Life Sciences Building
Northeastern University
360 Huntington Ave
Boston, MA 02115

Email: k.lewis@neu.edu
Tel: 617.373.8238

 

 

Selected Publications

Nadja Leimer*, Xiaoqian Wu*, Yu Imai*, Madeleine Morrissette, Norman Pitt, Quentin Favre-Godal, Akira Iinishi, Samta Jain, Mariaelena Caboni, Inga V. Leus, Vincent Bonifay, Samantha Niles, Rachel Bargabos, Meghan Ghiglieri, Rachel Corsetti, Megan Krumpoch, Gabriel Fox, Sangkeun Son, Dorota Klepacki, Yury S. Polikanov, Cecily Freliech, Julie McCarthy, Diane G. Edmondson, Steven J. Norris, Anthony D’Onofrio, Linden Hu, Helen I. Zgurskaya, Kim Lewis. (2021) A Selective Antibiotic for Lyme Disease. CELL (in press).

Manuse S*, Shan Y*, Canas-Duarte SJ*, Bakshi S, Sun WS, Mori H, Paulsson J, Lewis K. (2021) Bacterial persisters are a stochastically formed subpopulation of low-energy cells. PLoS Biology. 19(4):e3001194. PubMed / PLoS Biology

Kaur H*, Jakob RP*, Marzinek JK, Green R, Imai Y, Bolla JR, Agustoni E, Robinson CV, Bond PJ, Lewis K, Maier T, Hiller S. (2021) The antibiotic darobactin mimics a β-strand to inhibit outer membrane insertase. Nature. 593(7857):125-129. PubMed / Nature

Morrissette M*, Pitt N*, González A, Strandwitz P, Caboni M, Rebman AW, Knight R, D’Onofrio A, Aucott JN, Soloski MJ, Lewis K. (2020) A Distinct Microbiome Signature in Posttreatment Lyme Disease Patients. mBio. 11:e02310-20. PubMed / mBio

Quigley J, Peoples A, Sarybaeva A, Hughes D, Ghiglieri M, Achorn C, Desrosiers A, Felix C, Liang L, Malveira S, Millett W, Nitti A, Tran B, Zullo A, Anklin C, Spoering A, Ling LL, Lewis K. (2020) Novel Antimicrobials from Uncultured Bacteria Acting against Mycobacterium tuberculosis. mBio. 11(4):e01516-20. PubMed / mBio

Lewis K. (2020) The Science of Antibiotic Discovery. Cell. 181(1):29-45. PubMed / Cell

Imai Y*, Meyer KJ*, Iinishi A, Favre-Godal Q, Green R, Manuse S, Caboni M, Mori M, Niles S, Ghiglieri M, Honrao C, Ma X, Guo J, Makriyannis A, Linares-Otoya L, Böhringer N, Wuisan ZG, Kaur H, Wu R, Mateus A, Typas A, Savitski MM, Espinoza JL, O’Rourke A, Nelson KE, Hiller S, Noinaj N, Schäberle TF, D’Onofrio A, and Lewis K. (2019) A new antibiotic selectively kills Gram-negative pathogens. Nature. 576(7787):459-464. Full-text access / PubMed / Nature

Zalis EA*, Nuxoll AS*, Manuse S, Clair G, Radlinski LC, Conlon BP, Adkins J, Lewis K. (2019) Stochastic Variation in Expression of the Tricarboxylic Acid Cycle Produces Persister Cells. mBio. e01930-19. PubMed / mBio

Strandwitz P, Kim KH, Terekhova D, Liu JK, Sharma A, Levering J, McDonald D, Dietrich D, Ramadhar TR, Lekbua A, Mroue N, Liston C, Stewart EJ, Dubin MJ, Zengler K, Knight R, Gilbert JA, Clardy J, Lewis K. (2018) GABA-modulating bacteria of the human gut microbiota. Nat Microbiol. 4(3):396-403. PubMed / Nat Microbiol

Fenn K*, Strandwitz P*, Stewart EJ, Dimise E, Rubin S, Gurubacharya S, Clardy J, Lewis K. (2017) Quinones are growth factors for the human gut microbiota. Microbiome. 5(1):161. PubMed / Microbiome

Shan Y*, Brown Gandt A*, Rowe SE, Deisinger JP, Conlon BP, Lewis K. (2017) ATP dependent persister formation in Escherichia coli. mBio 8:e02267-16. PubMed / mBio

Conlon B, Rowe SE, Brown Gandt A, Nuxoll A, Donegan N, Zalis E, Clair G, Adkins J, Cheung A, Lewis K. (2016) Persister formation in Staphylococcus aureus is associated with ATP depletion. Nat Microbiol. 1:16051. PubMed / Nat Microbiol

Schumacher, M., Balani, P., Min, J., Chinnam, N., Hansen, S., Vulić, M., Lewis, K.*, Brennan, R.* (2015) HipAB-promoter structures reveal basis of heritable multidrug tolerance. Nature. 524:59-64. PubMed / Nature

Ling, L., Schneider, T., Peoples, A., Spoering, A., Engels, I., Conlon, B., Mueller, A., Schäberle, T., Hughes, D., Epstein, S., Jones, M., Lazarides, L., Steadman, V., Cohen, D., Felix C., Fetterman, K., Millett, W., Nitti, A.,Zullo, A., Chen, C., and Lewis, K. (2015) A novel antibiotic kills pathogens without detectable resistance. Nature. 517:455-459. PubMed / Nature

Gavrish, E., C. S. Sit, S. Cao, O. Kandror, A. Spoering, A. Peoples, L. Ling, A. Fetterman, D. Hughes, A. Bissell, H. Torrey, T. Akopian, A. Mueller, S. Epstein, A. Goldberg, J. Clardy and K. Lewis. (2014) Lassomycin, a ribosomally synthesized cyclic peptide, kills Mycobacterium tuberculosis by targeting the ATP-dependent protease ClpC1P1P2. Chem Biol. 21: 509-518. PubMed / Chem Biol

Conlon, B., Nakayasu, E., Fleck, L., LaFleur, M., Isabella, V., Coleman, K., Leonard, S., Smith, R., Adkins, J. and Lewis, K. (2013) Activated ClpP kills persisters and eradicates a chronic biofilm infection. Nature. 503(7476):365-370. PubMed / Nature

Keren, I., Wu, Y., Innocencio, J., Mulcahy, L., and Lewis, K. (2013) Killing by bactericidal antibiotics does not depend on reactive oxygen species. Science. 339:1213-1216. PubMed / Science