Our laboratory studies persister cells responsible for tolerance to antibiotics, uncultured bacteria of the environment and the microbiome, and works on drug discovery.


Kim Lewis, Ph.D.




Room 306C Mugar Life Sciences Building
Northeastern University
360 Huntington Ave
Boston, MA 02115

Email: k.lewis@neu.edu
Tel: 617.373.8238


Selected Publications

Strandwitz P, Kim KH, Terekhova D, Liu JK, Sharma A, Levering J, McDonald D, Dietrich D, Ramadhar TR, Lekbua A, Mroue N, Liston C, Stewart EJ, Dubin MJ, Zengler K, Knight R, Gilbert JA, Clardy J, Lewis K. (2018) GABA-modulating bacteria of the human gut microbiota. Nat Microbiol. 4(3):396-403. PubMed / Nat Microbiol

Wu X, Sharma B, Niles S, O’Connor K, Schilling R, Matluck N, D’Onofrio A, Hu LT, Lewis K. (2018) Identifying Vancomycin as an effective antibiotic for killing Borrelia Burgdorferi. Antimicrob. Agents Chemother. e01201-18. PubMed / Antimicrob Agents Chemother

Fenn K*, Strandwitz P*, Stewart EJ, Dimise E, Rubin S, Gurubacharya S, Clardy J, Lewis K. (2017) Quinones are growth factors for the human gut microbiota. Microbiome. 5(1):161. PubMed / Microbiome

Shan Y*, Brown Gandt A*, Rowe SE, Deisinger JP, Conlon BP, Lewis K. (2017) ATP dependent persister formation in Escherichia coli. mBio 8:e02267-16. PubMed / mBio

Conlon B, Rowe SE, Brown Gandt A, Nuxoll A, Donegan N, Zalis E, Clair G, Adkins J, Cheung A, Lewis K. (2016) Persister formation in Staphylococcus aureus is associated with ATP depletion. Nat Microbiol. 1:16051. PubMed / Nat Microbiol

Schumacher, M., Balani, P., Min, J., Chinnam, N., Hansen, S., Vulić, M., Lewis, K.*, Brennan, R.* (2015) HipAB-promoter structures reveal basis of heritable multidrug tolerance. Nature. 524:59-64. PubMed / Nature

Ling, L., Schneider, T., Peoples, A., Spoering, A., Engels, I., Conlon, B., Mueller, A., Schäberle, T., Hughes, D., Epstein, S., Jones, M., Lazarides, L., Steadman, V., Cohen, D., Felix C., Fetterman, K., Millett, W., Nitti, A.,Zullo, A., Chen, C., and Lewis, K. (2015) A novel antibiotic kills pathogens without detectable resistance. Nature. 517:455-459. PubMed / Nature

Gavrish, E., C. S. Sit, S. Cao, O. Kandror, A. Spoering, A. Peoples, L. Ling, A. Fetterman, D. Hughes, A. Bissell, H. Torrey, T. Akopian, A. Mueller, S. Epstein, A. Goldberg, J. Clardy and K. Lewis. (2014) Lassomycin, a ribosomally synthesized cyclic peptide, kills Mycobacterium tuberculosis by targeting the ATP-dependent protease ClpC1P1P2. Chem Biol. 21: 509-518. PubMed / Chem Biol

Conlon, B., Nakayasu, E., Fleck, L., LaFleur, M., Isabella, V., Coleman, K., Leonard, S., Smith, R., Adkins, J. and Lewis, K. (2013) Activated ClpP kills persisters and eradicates a chronic biofilm infection. Nature. 503(7476):365-370. PubMed / Nature

Keren, I., Wu, Y., Innocencio, J., Mulcahy, L., and Lewis, K. (2013) Killing by bactericidal antibiotics does not depend on reactive oxygen species. Science. 339:1213-1216. PubMed / Science

Dörr, T., Vulić, M., Lewis, K. (2010) Ciprofloxacin Causes Persister Formation by Inducing the TisB toxin in Escherichia coli. PLoS Biol. e1000317. PubMed / PLoS Biol

D’Onofrio, A., Crawford, J., Stewart, E., Witt, K., Gavrish, E., Epstein, S., Clardy, J., Lewis, K. (2010) Siderophores from Neighboring Organisms Promote the Growth of Uncultured Bacteria. Chem Biol. 17(3):254-264. PubMed / Chem Biol