Our laboratory studies persister cells responsible for tolerance to antibiotics, uncultured bacteria of the environment and the microbiome, and works on drug discovery.

 

Kim Lewis, Ph.D.

Contact

Room 306C Mugar Life Sciences Building
Northeastern University
360 Huntington Ave
Boston, MA 02115

Email: k.lewis@northeastern.edu
Tel: 617.373.8238

Join the Lewis Lab.

View openings for Postdoctoral Research Associate and Senior Research Technician positions.

 

Selected Publications

Yu Imai, Glenn Hauk, Jeffrey Quigley, Libang Liang, Sangkeun Son, Meghan Ghiglieri, Michael F. Gates, Madeleine Morrissette, Negar Shahsavari, Samantha Niles, Donna Baldisseri, Chandrashekhar Honrao, Xiaoyu Ma, Jason J. Guo, James M. Berger and Kim Lewis. (2022) Evybactin is a DNA gyrase inhibitor that selectively kills Mycobacterium tuberculosis. Nature Chemical Biology. full-text

R. Shukla, F. Lavore, S. Maity, M.G.N. Derks, C.R. Jones, B.J.A. Vermeulen, A. Melcrová, M.A. Morris, L.M Becker, X. Wang, R. Kumar, J. Medeiros-Silva, R.A.M. van Beekveld, A.M.J.J. Bonvin, J. Lorent, M. Lelli, J. Nowick, H.D. MacGillavry, A.J. Peoples, A.L. Spoering, L.L. Ling, D.E. Hughes, W.H. Roos, E. Breukink, K. Lewis, and M. Weingarth (2022) Teixobactin kills bacteria by a two-pronged attack on the cell envelope. Nature (Published online: 03 August 2022).full-text

Nadja Leimer*, Xiaoqian Wu*, Yu Imai*, Madeleine Morrissette, Norman Pitt, Quentin Favre-Godal, Akira Iinishi, Samta Jain, Mariaelena Caboni, Inga V. Leus, Vincent Bonifay, Samantha Niles, Rachel Bargabos, Meghan Ghiglieri, Rachel Corsetti, Megan Krumpoch, Gabriel Fox, Sangkeun Son, Dorota Klepacki, Yury S. Polikanov, Cecily Freliech, Julie McCarthy, Diane G. Edmondson, Steven J. Norris, Anthony D’Onofrio, Linden Hu, Helen I. Zgurskaya, Kim Lewis. (2021) A Selective Antibiotic for Lyme Disease. Cell. full-text, October 6, 2021.

Manuse S*, Shan Y*, Canas-Duarte SJ*, Bakshi S, Sun WS, Mori H, Paulsson J, Lewis K. (2021) Bacterial persisters are a stochastically formed subpopulation of low-energy cells. PLoS Biology. 19(4):e3001194. PubMed / PLoS Biology

Kaur H*, Jakob RP*, Marzinek JK, Green R, Imai Y, Bolla JR, Agustoni E, Robinson CV, Bond PJ, Lewis K, Maier T, Hiller S. (2021) The antibiotic darobactin mimics a β-strand to inhibit outer membrane insertase. Nature. 593(7857):125-129. PubMed / Nature

Morrissette M*, Pitt N*, González A, Strandwitz P, Caboni M, Rebman AW, Knight R, D’Onofrio A, Aucott JN, Soloski MJ, Lewis K. (2020) A Distinct Microbiome Signature in Posttreatment Lyme Disease Patients. mBio. 11:e02310-20. PubMed / mBio

Quigley J, Peoples A, Sarybaeva A, Hughes D, Ghiglieri M, Achorn C, Desrosiers A, Felix C, Liang L, Malveira S, Millett W, Nitti A, Tran B, Zullo A, Anklin C, Spoering A, Ling LL, Lewis K. (2020) Novel Antimicrobials from Uncultured Bacteria Acting against Mycobacterium tuberculosis. mBio. 11(4):e01516-20. PubMed / mBio

Lewis K. (2020) The Science of Antibiotic Discovery. Cell. 181(1):29-45. PubMed / Cell

Imai Y*, Meyer KJ*, Iinishi A, Favre-Godal Q, Green R, Manuse S, Caboni M, Mori M, Niles S, Ghiglieri M, Honrao C, Ma X, Guo J, Makriyannis A, Linares-Otoya L, Böhringer N, Wuisan ZG, Kaur H, Wu R, Mateus A, Typas A, Savitski MM, Espinoza JL, O’Rourke A, Nelson KE, Hiller S, Noinaj N, Schäberle TF, D’Onofrio A, and Lewis K. (2019) A new antibiotic selectively kills Gram-negative pathogens. Nature. 576(7787):459-464. Full-text access / PubMed / Nature

Strandwitz P, Kim KH, Terekhova D, Liu JK, Sharma A, Levering J, McDonald D, Dietrich D, Ramadhar TR, Lekbua A, Mroue N, Liston C, Stewart EJ, Dubin MJ, Zengler K, Knight R, Gilbert JA, Clardy J, Lewis K. (2018) GABA-modulating bacteria of the human gut microbiota. Nat Microbiol. 4(3):396-403. PubMed / Nat Microbiol

Fenn K*, Strandwitz P*, Stewart EJ, Dimise E, Rubin S, Gurubacharya S, Clardy J, Lewis K. (2017) Quinones are growth factors for the human gut microbiota. Microbiome. 5(1):161. PubMed / Microbiome

Shan Y*, Brown Gandt A*, Rowe SE, Deisinger JP, Conlon BP, Lewis K. (2017) ATP dependent persister formation in Escherichia coli. mBio 8:e02267-16. PubMed / mBio

Conlon B, Rowe SE, Brown Gandt A, Nuxoll A, Donegan N, Zalis E, Clair G, Adkins J, Cheung A, Lewis K. (2016) Persister formation in Staphylococcus aureus is associated with ATP depletion. Nat Microbiol. 1:16051. PubMed / Nat Microbiol

Schumacher, M., Balani, P., Min, J., Chinnam, N., Hansen, S., Vulić, M., Lewis, K.*, Brennan, R.* (2015) HipAB-promoter structures reveal basis of heritable multidrug tolerance. Nature. 524:59-64. PubMed / Nature

Ling, L., Schneider, T., Peoples, A., Spoering, A., Engels, I., Conlon, B., Mueller, A., Schäberle, T., Hughes, D., Epstein, S., Jones, M., Lazarides, L., Steadman, V., Cohen, D., Felix C., Fetterman, K., Millett, W., Nitti, A.,Zullo, A., Chen, C., and Lewis, K. (2015) A novel antibiotic kills pathogens without detectable resistance. Nature. 517:455-459. PubMed / Nature

Conlon, B., Nakayasu, E., Fleck, L., LaFleur, M., Isabella, V., Coleman, K., Leonard, S., Smith, R., Adkins, J. and Lewis, K. (2013) Activated ClpP kills persisters and eradicates a chronic biofilm infection. Nature. 503(7476):365-370. PubMed / Nature

Keren, I., Wu, Y., Innocencio, J., Mulcahy, L., and Lewis, K. (2013) Killing by bactericidal antibiotics does not depend on reactive oxygen species. Science. 339:1213-1216. PubMed / Science