Color Segmentation of Benthic Transects

Five Macintosh II systems were provided to the Marine Science Center by an Instruments and Instrument Development grant and other funds to implement a Biological Imaging laboratory consisting of hybrid video/computer motion analysis system, a video microscope, a macrophotography system, a slide scanner system and a flatbed scanner system. These instruments are available for general use by faculty and students of MSC and the Biology Department.

Correlated Electrophysiological and Kinematic Analysis of Animal Behavior

• A Motion Analysis System includes a Macintosh 7600 PPC with 32MB CPU Memory and a 1200Meg hard disk, Mitsubishi CP-10U Color Video Printer, and a Sony MVR-5500 Electronic Still Video Recorder/Player, Sony EVS-3000 High-8, and SLV-R5 SVHS decks as well as a CCD-V5000 camcorder.
  
• A Slide Scanning System includes a Macintosh IIci with a 1.2G Quantum Drive, a RasterOps 24STV Frame Grabber and a Sony PHV-A7 Photo Video slide camera.
  
• A Macrophotography System includes a Macintosh IIfx with 20MB processor memory, 240 MB Quantum hard disk, Syquist Backup Disk, Apple CD ROM Drive, two color and one monochrome displays, GW Instruments MacAdios II analog interface, Data Translation QuickCapture , RasterOps 24STV Frame Grabber,, Sony XC-211 RGB camera head and a DataTranslation QuickCapture interfaced for RGB acquisition. This system is interfaced to a Bogen Macrophotography System and an Olympus SZH10 Research Stereo Microscope.
  
  
  Segmentation and Analysis of Image Objects
• A Video Microscope System has a Macintosh IIx Processor with 8 MByte Processor Memory, a 240 MByte APS Hard Drive, DataTranslation Quick Capture Frame Grabber interfaced to a Javelin RGB camera head or a Sony XC-77 gray scale head, Varispec Tunable Filter which allows program selectable switching from 400-720 nM and an Olympus VANOX Microscope with Nomarski and Phase Contrast Systems.
  
• A Flatbed Scanner System includes Macintosh IIx with 8 MByte Processor Memory, a 240 MByte APS Hard Drive, an Apple Color Flatbed Scanner and associated software including OmniPage Pro OCR Software.
  

Visualization of Particle Flow

Software has been developed at MSC (ColorImage) which allows color based image segmentation and object quantification, as well as computer controlled stop-frame video based motion analysis (Ayers and Fletcher, 1991; Ayers, 1992) and is supported on all these systems.

New Features of ColorImage 1.59

• It operates with virtual memory under system 7.5.5
• Supports the full functionality of NIH Image 1.59
• RGB & Motion Analysis from Stacks
• Support for RasterOps 24STV/QT Vdig Color Movies
• Multi-Parameter Time-Based Kinematic Analysis from Movies
• Support for Vari-Spec Tunable Filter
• Support for Visualization of Particle Flow
• Support for Video Toolkit Videotape Logs and searches

ColorImage 1.59 Manual

ColorImage 1.59 (68K) Download (Major Bug Fix 4/19/97)

ColorImage 1.59 (PPC) Download (still Unstable 4/19/97)

• Ayers, J and Fletcher, G. (1990) Color Segmentation and Motion Analysis of Biological Image Data on the Macintosh II. Advanced Imaging 5: 39-42
• Ayers, J. (1992) Desktop Motion Video for Scientific Image Analysis. Advanced Imaging 7: 52-55.
• Breithaupt, T. and Ayers, J. (1996) Visualization and quantative analysis of underwater biological flow fields using suspended particles. Marine Behavior and Physiology, in press
• Maney, E.J., Ayers, J., Sebens, K.P, and Witman, J. (1990) Quantitative Techniques for Underwater Video Photography. In: Diving For Science. Am. Acad. Underwater Sci., St. Petersburgh. Pp. 255-265